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KMID : 0613820090190020228
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Journal of Life Science
2009 Volume.19 No. 2 p.228 ~ p.233
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Antioxidant Enzyme Activities in Tissues of Papilio xuthus during Pupal Stage
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Shin Myung-Ja
Kim Kyoung-Keun
Lim Jae-Hwan
Jeong Hyung-Jin
Seo Eul-Won
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Abstract
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The purpose of this study is to evaluate the activities of five different antioxidant enzymes in various tissues of Papilio xuthus during pupal stage. Superoxide dismutase (SOD) activity in haemolymph was the highest just after pupation and then decreased gradually until 7 days after pupation but the activity in other tissue was constant during metamorphosis. This result indicates that primary antioxidant process of reactive oxygen species proceed in haemolymph. Catalase (CAT) activity in studied tissues was also the highest just after pupation and its relative activity was also high during pupal stage, suggesting that CAT is the primary enzyme in catalysis of hydrogen peroxide. Glutathion peroxidase (GPX) activity was constant and its relative activity was very low in all tested tissues. Glutathione S-transferase (GST) activity in haemolymph was high at 3 days and 5 days after pupation, and the activity in fat body was the highest at the 1 day after pupation and then decreased gradually for 7 days after pupation. Glutathion reductase (GR) activity in haemolymph and fat body was high at 1 day after pupation, but relatively low GR activity was detected in the rest tissues. Based on these results, GST activity was higher than that of GPX and GR, and it is also believed that GST was more involved in reduction process through lipid peroxidation than GPX.
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KEYWORD
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Antioxidant enzyme, tissue, pupal stage, Papilio xuthus
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